Regulation of human ornithine decarboxylase expression by the c-Myc.Max protein complex.
作者： A Peña , C D Reddy , S Wu , N J Hickok , E P Reddy
DOI: 10.1016/S0021-9258(19)74246-X
关键词: Methylation 、 Ornithine decarboxylase 、 Transfection 、 Messenger RNA 、 Transcription (biology) 、 Molecular biology 、 Biology 、 Regulation of gene expression 、 Transcriptional regulation 、 Electrophoretic mobility shift assay
摘要: The presence of a CACGTG element within region the human ornithine decarboxylase (ODC) promoter located at -491 to -474 base pairs 5' start site transcription suggested that c-Myc.Max protein complex may play role in regulation ODC expression during growth. Electrophoretic mobility shift assays and methylation interference analysis showed nuclei WI-38 cells expressing contained proteins bound this gene manner correlated with growth-associated expression. Also, use antibodies against c-Myc Max purified recombinant electrophoretic assay confirmed these can specifically bind portion promoter. Transient transfection studies increase level and/or led significant enhancement promoter-CAT reporter construct. Moreover, treatment actively growing an antisense oligomer reduced amount endogenous formed mRNA expressed. These show plays transcriptional vivo.
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