Elution of low molecular weight solutes from viable cells of Saccharomyces bisporus

摘要: Previous work has shown that high molecular weight compounds were released from Saccharomyces bisporus by β-mercaptoethanol, 2 M KCl, 0.5 KCl and osmotic shock without affecting viability of the cells. In this current experiment, it was low also eluted when cells treated in sequence with same reagents. Alanine, glutamate, serine, an unidentified amino acid, glucose, glycerol, arabitol all each first three The eluate contained a larger number quantity acids than eluates but, otherwise, same. One hundred percent cellular glycerol 65–70% total amounts other above mentioned solutes 4 eluting treatments. A hot water treatment needed to extract remainder these solutes. almost proline. It suggested elutable are compartments (or vesicles) whose membranes accessible reagents plasmalemma.