A Combination of Isocratic and Gradient Elution Modes in HPLC with the Aid of Time-Overlapping Process for Rapid Determination of Methyldopa in Human Urine
作者： Samy Emara , Tsutomu Masujima , Walaa Zarad , Maha Kamal , Marwa Fouad
DOI: 10.1080/10826076.2014.896813
关键词: Methyldopa 、 Chemistry 、 Urine 、 High-performance liquid chromatography 、 Calibration curve 、 Gradient elution 、 Buffer solution 、 Acetonitrile 、 Scientific method 、 Chromatography 、 Analytical chemistry
摘要: A new rapid time-overlapping high-performance liquid chromatography method using coupled-column double-injection technique with fluorescence detection has been developed and validated to determine methyldopa (MTD) in human urine. The was based on injecting a sample onto the second column before finalizing cleanup re-equilibration of first for former sample. combination isocratic gradient elution employed according pre-set program. At beginning, step acetate buffer solution (0.1 M, pH 2.4) set until 7 min. Subsequently, as mobile phase acetonitrile B employed. After end each step, re-equilibrated 4 mL starting system next analysis. overall cycle time 7 min per calibration curve linear over concentration range 0.1–40 µg/mL MTD. ...
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