Novel binding specificities engineered into the scaffold of a carbohydrate binding module

摘要: The growing field of biotechnology is in constant need proteins that can function as recognition tools for separational, analytical and therapeutic purposes. Different molecular engineering approaches are applied on natural order to create variants with desired properties. This thesis based five original papers deal selection, characterisation application novel binding specificities engineered into the scaffold a carbohydrate module originates from xylanase thermophilic bacterium Rhodothermus marinus. Molecular evolution studies this allowed generation bind specifically targets xylan, Avicel?, mannan xyloglucan. In addition, employed work was also able adopt specific protein human IgG4 molecule. Apart high specificities, have additional properties such thermal stability ease production Escherichia coli, which advantageous most applications. One demonstrates potential use created xylan-binding detection their target wood fibres plant sections. Also, generated other find similar bioanalytical tools. conclusion, engaged proved be suitable carrying diversity has thus creation diverse useful biotechnological