Validation of Cryo-EM Structure of IP3R1 Channel

摘要: About a decade ago, three electron cryomicroscopy (cryo-EM) single-particle reconstructions of IP3R1 were reported at low resolution. It was disturbing that these structures bore little similarity to one another, even the level quaternary structure. Recently, we published an improved structure ∼1 nm However, this did not bear any resemblance previously structures, leading question why should be considered more reliable than original three. Here, apply several methods, including class-average/map comparisons, tilt-pair validation, and use multiple refinement software packages, give strong evidence for reliability our recent The map resolution feature resolvability are assessed with gold standard criterion. This approach is generally applicable assessing validity cryo-EM maps other molecular machines.