Detection of pregnancy in sheep by radioimmunoassay of sera for pregnancy-specific protein B.

作者: C.A. Ruder , J.N. Stellflug , J.J. Dahmen , R.G. Sasser

DOI: 10.1016/0093-691X(88)90227-0

关键词: PregnancyBiologyUltrasonic deviceSpecific proteinRadioimmunoassayEndocrinologyInternal medicineAnimal science

摘要: A radioimmunoassay (RIA) for bovine pregnancy-specific protein B (bPSPB) has been shown to be a reliable test pregnancy in cows. Pregnant ewes have blood antigen that cross-reacts this RIA. Two studies were conducted determine the accuracy of detection sheep using bPSPB In Study 1, 33 ewe lambs bred over 70-d period late fall. At 26, 56, and 83 d after end breeding period, samples collected assay RIA, Pregmatic 3 ultrasonic device was used detect pregnancy. detected 14, 27 28 nonpregnancy 19, 6 at Days 56 past respectively. The 32, 31 30 2 breeding, respectively, Thirty lambed three did not. 2, 180 multiparous 60-d summer. 35 real-time scan done Real-time testing 163 17 ewes; whereas, RIA 161 19 ewes. One hundred fifty-nine 21 earlier more accurately than equally as accurate scanning instrument. These demonstrate an serological sheep.

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