Pro-myogenic and low-oxygen culture increases expression of contractile smooth muscle markers in human fibroblasts.

摘要: Smooth muscle cells are essential for tissue engineering strategies to fabricate organs such as blood vessels, esophagus, and bladder, create disease models of these systems. In order therapies be feasible, smooth must sourced effectively enable production large numbers functional cells. vitro, divide slowly demonstrate short proliferative lifespans compared other types including stem fibroblasts, limiting the number that can derived from expansion in culture a primary isolation. As such, it would beneficial better understand factors underlying induction maintenance cell phenotypes, produce new sources modeling. Here we report ability human dermal fibroblasts display patterns gene expression resembling contractile when cultured under conditions known promote phenotype cells, on collagen IV, low serum culture, TGF-β1 treatment, hypoxia. These drive myogenic transcription factor myocardin, well several its targets contributors alpha actin, calponin, myosin heavy chain. Our results suggest associated with may sufficient induce potential function non-muscle This article is protected by copyright. All rights reserved.