De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis

摘要: Abstract Plukenetia volubilis is a promising oilseed crop due to its seeds being rich in unsaturated fatty acids, especially alpha-linolenic acid. P. monoecious, with separate male and female flowers on the same inflorescence. We previously reported that were converted by exogenous cytokinin (6-benzyladenine, 6-BA) treatment . To identify candidate genes associated floral sex differentiation of , we performed de novo transcriptome assembly comparative analysis control inflorescence buds (MIB) (FIB) induced 6-BA using Illumina sequencing technology. A total 57,664 unigenes an average length 979 bp assembled from 104.1 million clean reads, 45,235 (78.45%) successfully annotated public databases. Notably, Gene Ontology analyses revealed 4193 3880 enriched categories reproduction reproductive processes, respectively. Differential expression identified 1385 differentially expressed between MIB FIB, which six related auxin signaling pathways 16 important transcription factor (TF) including MADS-box family members identified. In particular, several encoding TFs, such as homologs CRABS CLAW, RADIALIS-like 1, 2, HECATE WUSCHEL-related homeobox 9, SUPERMAN, at higher levels FIB than MIB. The patterns 36 selected validated quantitative real-time PCR. This study not only provides comprehensive gene profiles buds, but also lays foundation for research molecular mechanism determination other monoecious plants.