Unnatural ligands for engineered proteins: new tools for chemical genetics.

摘要: ▪ Abstract Small molecules that modulate the activity of biological signaling can be powerful probes signal transduction pathways. Highly specific with high affinity are difficult to identify because conserved nature many protein active sites. A newly developed approach discovery such small relies on engineering and chemical synthesis has yielded tools for study a wide variety proteins involved in (G-proteins, kinases, 7-transmembrane receptors, nuclear hormone others). Such genetic combine advantages traditional genetics unparalleled temporal control over function afforded by molecule inhibitors/activators act at diffusion controlled rates targets.