Effects of ozone exposure in rat lungs investigated with hyperpolarized 3 He MRI.

摘要: Purpose To investigate the effects of subchronic ozone exposure on rat lung ventilation using hyperpolarized (HP) 3He MRI. Materials and Methods A total 24 Sprague-Dawley rats, distributed in one control group four groups exposed to 0.5 ppm concentration for two days or six days, either continuously (22 hours/day) alternatingly (12 hours/day). A three-step MRI protocol was designed applied each animal, including: 1) gas distribution images acquired at inspiratory capacity, 2) measurements intrapulmonary diffusion coefficients, 3) dynamic acquisitions performed during filling with 3He. Results No differentiation between animals observed from obtained capacity. The coefficients were not statistically different another. Ventilation defects, appearing as delayed regions heterogeneous filling, image series. percentage defects control, two-day, six-day equal 20%, 43% 75%, respectively. In subgroup 12 hours per day, exhibiting 85%. Conclusion Heterogeneous obstructive patterns an experimental animal model HP MRI. J. Magn. Reson. Imaging 2008;27:771–776. © 2008 Wiley-Liss, Inc.