Microglial cell cytotoxicity of oligodendrocytes is mediated through nitric oxide.
作者： Thomas E. Lane , Jean E. Merrill , Louis J. Ignarro , Michael P. Sherman , Judy Melinek
DOI:
关键词: Biochemistry 、 Nitric oxide 、 Cell biology 、 In vitro 、 Biology 、 Oligodendrocyte 、 Programmed cell death 、 Microglia 、 Antibody 、 Intracellular 、 Cytotoxicity
摘要: Rat ameboid microglia are able to lyse rat oligodendrocytes in vitro. The lysis is inhibited by transforming growth factor-beta, antagonists of nitric oxide (NO) production, as well antibodies TNF-alpha, intercellular adhesion molecule-1 (ICAM-1), and leukocyte functional Ag-1. Ameboid microglial cells spontaneously produce detectable levels the NO metabolite nitrite (NO2-). Stimuli such PMA, LPS, and/or IFN-gamma induce micromolar concentrations NO2- within 24 h. TNF-alpha increases IFN gamma but not LPS-induced production. Incubation with target also production a contact-dependent manner. synthase antagonists, anti TNF-alpha. Neither antileukocyte Ag-1 nor anti-ICAM-1 inhibit presence or absence oligodendrocytes. Indeed, treatment There correlation between cell killing suggesting may be mechanism death oligodendrocyte possibly play role lesion formation multiple sclerosis.
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