The CpxA/CpxR Two-Component System Affects Biofilm Formation and Virulence in Actinobacillus pleuropneumoniae

摘要: Gram-negative bacteria have evolved numerous two-component systems (TCSs) to cope with external environmental changes. The CpxA/CpxR TCS consisting of the kinase CpxA and regulator CpxR, is known be involved in biofilm formation virulence Escherichia coli. However, role remained unclear Actinobacillus pleuropneumoniae, a bacterial pathogen that can cause porcine contagious pleuropneumonia (PCP). In this report, we show contributes ability A. pleuropneumoniae. Furthermore, demonstrate plays an important expression several biofilm-related genes such as rpoE pgaC. results electrophoretic mobility shift assays (EMSAs) DNase I footprinting analysis CpxR-P regulate pgaABCD operon through rpoE. experimental infection mice, animals infected cpxA/cpxR mutant exhibited delayed mortality lower loads lung than those wildtype bacteria. conclusion, these indicate contributing