Characterization of the low density lipoprotein receptor in membranes prepared from human fibroblasts.
作者： Joseph L Goldstein , Michael S Brown , S. K. Basu
DOI: 10.1016/S0021-9258(17)34768-3
关键词: Biology 、 Molecular biology 、 Cholesterol 、 Receptor 、 Pronase 、 Membrane 、 Low-density lipoprotein 、 LDL receptor 、 Familial hypercholesterolemia 、 High-density lipoprotein 、 Biochemistry
摘要: An ultracentrifugation assay has been developed to measure low density lipoprotein (LDL) receptor activity in membranes prepared from cultured human fibroblasts. The binding site for 125I-labeled LDL isolated reflected the properties of previously demonstrated intact It exhibited high affinity (Kd approximately 4 microgram protein/ml), specificity (LDL 400-fold more effective than competing with 125I-LDL site), dependence on calcium, and susceptibility destruction by pronase. number receptors detected vitro membrane was similar cells. reduced fibroblasts that were grown presence 25-hydroxycholesterol plus cholesterol fibroblast a subject homozygous familial hypercholesterolemia, two situations which is known be reduced. availability faithfully reflects physiologic cells should permit characterization this organs humans animals.
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