The effect of toxic pyridine-alkaloid secondary metabolites on the sunbird gut microbiome.

作者: Mohanraj Gunasekaran , Maya Lalzar , Yehonatan Sharaby , Ido Izhaki , Malka Halpern

DOI: 10.1038/S41522-020-00161-9

关键词: NicotineGut floraFood scienceLactococcusNectarBacteriaBiologyAlkaloidAnabasineSunbird

摘要: Sunbirds feed on tobacco tree nectar which contains toxic nicotine and anabasine secondary metabolites. Our aim was to understand the effect of gut microbiota composition sunbirds. Sixteen captive sunbirds were randomly assigned two diets: artificial either with (treatment) or without (control) added anabasine. Excreta collected at 0, 2, 4 7 weeks treatment samples processed for bacterial culture high-throughput amplicon sequencing 16S rRNA gene. The microbiome diversity treated control birds changed differently along seven-week experiment. While decreased in group first three samplings (0, 2 weeks), it increased group. analyses demonstrated that a diet anabasine, significantly birds' compared birds. abundance nicotine- anabasine- degrading bacteria excreta birds, higher after four seven Furthermore, analysis culturable isolates, including Lactococcus, showed sunbirds' gut-associated capable consistent their hypothesised role as detoxifying nutritional symbionts.

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