Locomotor Recovery in Spinal Cord-Injured Rats Treated with an Antibody Neutralizing the Myelin-Associated Neurite Growth Inhibitor Nogo-A
作者： Doron Merkler , Gerlinde AS Metz , Olivier Raineteau , Volker Dietz , Martin E Schwab
DOI: 10.1523/JNEUROSCI.21-10-03665.2001
关键词: Open field 、 Nociception 、 Neuroscience 、 Neutralizing antibody 、 Spinal cord 、 Motor system 、 Internal medicine 、 Tail flick test 、 Myelin 、 Endocrinology 、 Antibody 、 Medicine
摘要: The limited plastic and regenerative capabilities of axons in the adult mammalian CNS can be enhanced by application a monoclonal antibody (mAb), IN-1, raised against myelin-associated neurite growth inhibitor Nogo-A. aim present study was to investigate effects this treatment on functional recovery rats with dorsal over-hemisection spinal cord. Directly after injury, half animals were implanted mAb IN-1-secreting hybridoma cells, whereas others received cells secreting control (anti-HRP). A broad spectrum locomotor tests (open field locomotor) score, grid walk, misstep withdrawal response, narrow-beam crossing) used characterize during 5 weeks injury. In all behavioral tests, IN-1-treated group significantly augmented compared antibody-treated rats. EMG recordings flexor extensor muscles treadmill walking confirmed improvement pattern rats; step-cycle duration, rhythmicity, coupling hindlimbs improved. No differences between two groups regard nociception observed tail flick test operation. These results indicating improved suggest that increased Nogo-A neutralization result functionally meaningful rewiring motor systems.
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