Meconium impairs pulmonary surfactant by a combined action of cholesterol and bile acids.

摘要: Mechanisms for meconium-induced inactivation of pulmonary surfactant as part the meconium aspiration syndrome in newborn infants, to our knowledge, are not clearly understood. Here we have studied biophysical mechanisms how affects surface activity and whether membrane-perturbing effects can be mimicked by exposure a mixture bile acids cholesterol. Surface complexes purified from animal lungs was analyzed absence presence standard balances captive bubble surfactometer. We also evaluated accumulation at air-liquid interface what believe novel microtiter plate fluorescent assay, effect components on membrane fluidity using Laurdan fluorescence thermotropic profiles differential scanning calorimetry thermograms. Rapid interfacial adsorption, low tension upon film compression, efficient replenishment expansion, properties all adversely affected meconium, and, similar manner, they cholesterol/taurocholate mixtures but taurocholate alone. conclude that inhibition salt-promoted incorporation excess cholesterol into complexes. These results highlight potential pathogenic role cholesterol-mobilizing agents crucial factor resulting induced alterations structure dynamics membranes films.