Target-responsive vasoactive probes for ultrasensitive molecular imaging

摘要: ABSTRACT The ability to monitor molecules volumetrically throughout the body could provide valuable biomarkers for studies of healthy function and disease, but noninvasive detection molecular targets in living subjects often suffers from poor sensitivity or selectivity. Here we describe a family potent imaging probes that can be activated by interest deep tissue, providing basis mapping nanomolar-scale analytes without radiation heavy metal content associated with traditional agents. are reversibly-caged vasodilators induce responses detectable hemodynamic imaging; they constructed combining vasoactive peptides synthetic chemical appendages protein blocking domains. We use this architecture create ultrasensitive biotin-responsive agents, which apply wide-field rat brains using functional magnetic resonance imaging. also adapt sensor design detecting neurotransmitter dopamine, illustrating versatility approach addressing biologically important molecules.