Headspace-gas chromatography-mass spectrometry for the rapid determination of possible biomarkers in urine samples
作者： Rosa María González Paredes , Carmelo García Pinto , José Luis Pérez Pavón , Bernardo Moreno Cordero
DOI: 10.1039/C7AY01655G
关键词: Mass spectrometry 、 Gas chromatography 、 Chromatography 、 Urine 、 Reproducibility 、 Chemistry 、 Standard addition 、 Repeatability 、 Analyte 、 Detection limit
摘要: The development of rapid analytical strategies plays a vital role for the research, discovery and confirmation analytes that can be sensitive biomarkers. Within this line work, method based on headspace-gas chromatography-mass spectrometry has been developed validated determination possible biomarkers (dimethyl disulfide, toluene, 1,4-xylene, 2-octanone 2,6-dimethyl-7-octen-2-ol) in human urine samples. matrix effect was analyzed optimal conditions were determined. limits detection ranged from 0.01 to 0.48 μg L−1, whereas quantification 0.05 1.43 L−1. repeatability reproducibility proposed below 9.5% 14.2%, respectively. Five samples healthy subjects, spiked with biomarkers, used validate methodology. these compounds three subjects performed one-point standard additions protocol.
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