Regionally specific and rapid increases in brain-derived neurotrophic factor messenger RNA in the adult rat brain following seizures induced by systemic administration of kainic acid

摘要: Abstract In situ hybridization techniques were used to analyse the spatiotemporal pattern of brainderived neurotrophic factor messenger RNA elevation associated with kainic acid-induced seizure activity in rat. Pronounced increases hippocampal brain-derived levels observed as early 30 min following onset behavioral seizures. The greatest increase (10-fold) occurred dentate granule cell layer, while pyramidal layers CA1, CA3, and CA4 exhibited two- six-fold. Peak CA1 region was evident at 4 h layer postseizure. Elevations persisted hilar regions four days, CA3 returned control by 16 seizure. Significant also superficial cortex (II III) piriform which reached peak elevations 8 h. No detectable changes dorsomedial thalamus. Although histologically defined displayed relatively uniform response kainate, a closer examination labeling patterns using emulsion autoradiography revealed discrete areas high grain densities overlapping uniform, moderate suggesting that capacity upregulate these may differ among individual neurons. conclusion, our studies induction systemic kainate administration differs cortical areas, magnitude, time duration. temperospatial does not correspond simple way metabolic or electrical seizures neuronal vulnerability coincident