Effect of estrogen and progesterone on gene expression of growth regulatory molecules and proto-oncogene in vascular smooth muscle cells.
作者： TOMOHARU OKUBO , MAMORU URABE , HIROSHI TSUCHIYA , KOICHI IWASA , KAZUAKI YOKOTA
关键词: Estrogen 、 Oncogene 、 Progestogen 、 Gene expression 、 Internal medicine 、 Chemistry 、 Vascular smooth muscle 、 Platelet-derived growth factor receptor 、 Endocrinology 、 Growth factor 、 Messenger RNA
摘要: We examined influences of estrogen and progestogen on gene expression the growth regulatory molecules: platelet-derived factor (PDGF), interleukin-1 (IL-1), interleukin-6 (IL-6) proto-oncogene c- myc in vascular smooth muscle cells (VSMC) by reverse transcription-polymerase chain reaction (RT-PCR) Southern-blotting. VSMC were exposed to estrone-sulfate (E1-S) medroxyprogesterone acetate (MPA) induce differentiation. E1-S inhibited PDGF-A chain, IL-1, IL-6 c-myc mRNA, whereas MPA had no effect. Inhibition was not affected treatment combined with MPA. These findings suggest that modulates these molecules but progestogen. concluded may have a direct atheroprotective effect through inhibition factors.
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