Proteomic Analysis of Aortae from Human Lipoprotein(a) Transgenic Mice Shows an Early Metabolic Response Independent of Atherosclerosis
作者： Euan J Rodger , Rachel J Suetani , Gregory T Jones , Torsten Kleffmann , Alan Carne
DOI: 10.1371/JOURNAL.PONE.0030383
关键词: High-density lipoprotein 、 Genetically modified mouse 、 Immunology 、 Lipoprotein 、 Lipid metabolism 、 Biology 、 Downregulation and upregulation 、 Lipoprotein(a) 、 Internal medicine 、 Endocrinology 、 Cholesterol 、 Gel electrophoresis 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Background: Elevated low density lipoprotein (LDL) and lipoprotein(a) are independent risk factors for the development of atherosclerosis. Using a proteomic approach we aimed to determine early changes in arterial protein expression transgenic mice containing both human LDL circulation. Methods Results: Plasma lipid analyses showed had significantly higher levels than wildtype, including much increased high (HDL) cholesterol. Analysis aortae from accumulation but no or foam cells, leaving arteries essentially atherosclerosis free. two-dimensional gel electrophoresis mass spectrometry, identified 34 proteins with altered abundance (P<0.05) compared wildtype 17 that ≥2 fold difference. Some interest similarly at transcript level. These collectively indicated an initial metabolic response included down regulation energy, redox metabolism structural stage when not yet developed. Conclusions: Our study shows promote unique set which may be indicators disturbances preceding
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