Efficient platelet delta-granule release induced by [Ca2+]i elevation is modulated by GPIIbIIIa.

摘要: Intracellular Ca2+ elevation generates a cascade of events that leads to platelet activation and degranulation. The GPIIbIIIa-ligand molecular complex plays central role in several aspects activation. Taking advantage the flow cytometric simultaneous analysis surface GPIIbIIIa expression intracellular serotonin content, we demonstrate here functional inhibition an impairment delta-granule release even upon maximal elevation. In healthy subjects, inhibitor tirofiban impairs release. Analogously, Glanzmann thrombasthenia patients show is proportional their residual GPIIbIIIa. These data required for fully efficient secretion delta-granules implications our findings are discussed light interplay between vesicle ligand-receptor triggering during