Neutralization-sensitive epitopes are exposed on the surface of infectious Cryptosporidium parvum sporozoites.

摘要: Cryptosporidiosis is a diarrheal disease of humans, calves, and other mammals caused by the coccidian parasite Cryptosporidium parvum. Immune bovine serum two surface-reactive antisporozoite mAb with neutralizing activity were used to identify sporozoite surface Ag radioimmunoprecipitation/SDS-PAGE immunoblotting. When isolated sporozoites incubated 18.44, 12 25 times ID50 for mice was completely neutralized. This binds diffusely recognizes that eluted in void volume Bio Gel A column an exclusion limit 500,000 daltons. The recognized 18.44 not radiolabeled 125I or [35S] methionine, migrated dye front SDS-PAGE, insensitive proteinase K digestion, suggesting non-protein composition. 17.41 significantly neutralized mice. multifocally methionine-labeled 28,000 m.w., 55,000 98,000 m.w. immunoprecipitated methionine- 125I-labeled ranging from less than 14,300 greater 200,000 including results indicate different molecules capable inducing antibody are exposed on C. parvum sporozoites.