Assumed initiation site of fibroin gene transcription

摘要: Abstract To map the initiation site of fibroin gene transcription on cloned genomic DNA (pFb29), we looked for precursors mRNA, first by applying hybridization mapping method to RNA from posterior silk gland Bombyx mori. The 6.2-kilobase (kb) fragment used assay, 5.7 kb upstream 0.5 downstream cap site, was end-labeled at Xho I (position +514) within intervening sequence gene. By analyzing labeled protected nuclease S1 treatment, have shown that is transcribed together with surrounding sequences in (producer) but not middle (nonproducer). Large transcripts could include entire (1 kb) and mRNA (16 were found. 5' end such located around or near locus. Synthesis analysis complementary primed region also confirmed These results strengthen possibility