Metabolism of oxidized linoleic acid: characterization of 13-hydroxyoctadecadienoic acid dehydrogenase activity from rat colonic tissue
作者： Sonja M. Earles , Joel C. Bronstein , David L. Winner , Arthur W. Bull
DOI: 10.1016/0005-2760(91)90023-B
关键词: Oxoglutarate dehydrogenase complex 、 Biochemistry 、 13-Hydroxyoctadecadienoic acid 、 Linoleic acid 、 Alcohol dehydrogenase 、 Branched-chain alpha-keto acid dehydrogenase complex 、 Enzyme assay 、 Chemistry 、 Enzyme 、 Dehydrogenase 、 Biophysics 、 Endocrinology
摘要: An oxidized derivative of linoleic acid, 13-hydroxyoctadecadienoic acid (13-HODE), is dehydrogenated by an NAD+ dependent dehydrogenase present in rat colon mucosa. The product the reaction 2,4-dienone, 13-oxooctadecadienoic acid. Enzyme activity was determined HPLC analysis incubation mixtures as well measuring increase absorbance at 285 nm, which represents formation 2,4-dienone chromophore. Characteristics with respect to protein concentration, time and substrate dependence were investigated. Several inhibitors known dehydrogenases had no effect on 13-HODE dehydrogenase. These include, ethanol, indomethacin, 6-methyl-17-hydroxyprogesterone acetate, 4-(diethylamino)-benzaldehyde, aspirin. enzyme mildly inhibited pyrazole, 4-methylpyrazole ibuprofen. Disulfiram found be a potent inhibitor IC50 200 microM. Inhibitor specificity, other characteristics suggest neither alcohol dehydrogenase, diol nor prostaglandin It possible this plays important role response colonic mucosa mitogenic fatty acids.
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