Profile of Human TERT and P73 in Oral Squamous Cell Carcinoma Cell Lines Compared to Normal Human Oral Mucosa: a Preliminary Study
作者： Ambar Kusuma Astuti , Irfan Prasetyo , Justisia Nafsi Yunita , Yuniardini Septorini Wimardhani , Febrina Rahmayanti
关键词: Mutant 、 Pathology 、 Cancer cell 、 Oral mucosa 、 Molecular biology 、 Cell culture 、 Telomerase reverse transcriptase 、 Neoplastic transformation 、 Biology 、 Polyacrylamide gel electrophoresis 、 Telomerase
摘要: Genetic alteration on p53 allows cellular immortalization and predisposes cells to neoplastic transformation. This is related telomere length maintenance by telomerase. Human TERT a key compo-nent of telomerase, which activity suppressed p53. The p73, the homolog p53, has similar ability in tumor suppression. P73 expressed at different level various cancer normal tissues. Profile human mutant OSCC cell line oral mucosa have not been known. Objective: To observe profile lines mucosa. Methods: extracted protein HSC-3 HSC-4 mucosal tissues were ana-lyzed with SDS PAGE detect p73 expression based molecular weight. Results: showed thicker band density compared its HSC-4. Only 50% tissue thick density. was clearly shown but Conclusion: Different might be cell-type specific influenced status Analy-sis role these cancers need further research determine possible substitution for function. DOI: 10.14693/jdi.v18i1.55
sci-hub.st 参考文章(21)
SKS Kumar, Rosnah Binti Zain, SM Ismail, SC Cheong, None, Human telomerase reverse transcriptase expression in oral carcinogenesis--a preliminary report. Journal of Experimental & Clinical Cancer Research. ,vol. 24, pp. 639- 646 ,(2005)
David Bryder, Derrick J. Rossi, Irving L. Weissman, Hematopoietic stem cells: the paradigmatic tissue-specific stem cell American Journal of Pathology. ,vol. 169, pp. 338- 346 ,(2006) , 10.2353/AJPATH.2006.060312
Stein Sæbøe-Larssen, Ellen Fossberg, Gustav Gaudernack, Characterization of novel alternative splicing sites in human telomerase reverse transcriptase (hTERT): analysis of expression and mutual correlation in mRNA isoforms from normal and tumour tissues BMC Molecular Biology. ,vol. 7, pp. 26- 26 ,(2006) , 10.1186/1471-2199-7-26
R. Fujimoto, N. Kamata, K. Yokoyama, N. Ueda, K. Satomura, E. Hayashi, M. Nagayama, Expression of telomerase components in oral keratinocytes and squamous cell carcinomas Oral Oncology. ,vol. 37, pp. 132- 140 ,(2001) , 10.1016/S1368-8375(00)00073-7
Li Mao, Waun K Hong, Vassiliki A Papadimitrakopoulou, Focus on head and neck cancer Cancer Cell. ,vol. 5, pp. 311- 316 ,(2004) , 10.1016/S1535-6108(04)00090-X
M S Irwin, F D Miller, p73: regulator in cancer and neural development. Cell Death & Differentiation. ,vol. 11, ,(2004) , 10.1038/SJ.CDD.4401452
Y. Cong, The Human Telomerase Catalytic Subunit hTERT: Organization of the Gene and Characterization of the Promoter Human Molecular Genetics. ,vol. 8, pp. 137- 142 ,(1999) , 10.1093/HMG/8.1.137
Elsa R. Flores, Shomit Sengupta, John B. Miller, Jamie J. Newman, Roderick Bronson, Denise Crowley, Annie Yang, Frank McKeon, Tyler Jacks, Tumor predisposition in mice mutant for p63 and p73: Evidence for broader tumor suppressor functions for the p53 family Cancer Cell. ,vol. 7, pp. 363- 373 ,(2005) , 10.1016/J.CCR.2005.02.019
Dawei Xu, Qian Wang, Astrid Gruber, Magnus Björkholm, Zhiguo Chen, Ahmed Zaid, Galina Selivanova, Curt Peterson, Klas G Wiman, Pavel Pisa, Downregulation of telomerase reverse transcriptase mRNA expression by wild type p53 in human tumor cells Oncogene. ,vol. 19, pp. 5123- 5133 ,(2000) , 10.1038/SJ.ONC.1203890
Wen Hong Toh, Satoru Kyo, Kanaga Sabapathy, Relief of p53-mediated Telomerase Suppression by p73 Journal of Biological Chemistry. ,vol. 280, pp. 17329- 17338 ,(2005) , 10.1074/JBC.M500044200