Functional characterization of T cells differentiated in vitro from bone marrow-derived CD34 cells of psoriatic patients with family history.

摘要: Please cite this paper as: Functional characterization of T cells differentiated in vitro from bone marrow-derived CD34+ psoriatic patients with family history. Experimental Dermatology 2010; 19: e128–e135. Abstract Background:  The strong but complex genetic background suggests that inherent and intrinsic rather than exogenous factors have a key role immunopathogenesis psoriasis. It is reasonable to speculate the dysfunctional activity may partly originate abnormal haematopoietic cells. Objectives:  To test if originated progenitor psoriasis display functional alternations similar previously reported abnormalities circulating cells. Methods:  Bone marrow were isolated history healthy subjects, into thymic stromal co-culture system. These further subjected comparisons such as proliferation, secretion cytokines IL-4, IL-8 IFN–γ, inducing production C-myc, Bcl-xL, Ki67 proteins human keratinocytes. Results:  While both volunteers developed mature within weeks environment vitro, showed higher proliferation stronger capacity secret TH1 response streptococcal superantigen. patients, not normal controls, induced overexpression C-myc Ki67, Bcl-XL, keratinocytes. Conclusions:  are functionally cells, suggesting can be traced back early development cells.