miR-373-3p inhibits epithelial-mesenchymal transition via regulation of TGFβR2 in choriocarcinoma.

摘要: AIM Previous studies have indicated that early metastasis is a major cause of mortality in patients with choriocarcinoma. However, what determines whether choriocarcinoma has occurred unknown. The emerging role miRNA regulating cancer development and progression been recognized. miR-373 shown to play pivotal roles tumorigenesis metastasis. functions promote not clear. purpose this study determine the function miR-373-3p cancer. METHODS In study, we first compared epithelial-mesenchymal transition (EMT)-related markers, which were inversely correlated expression trophoblast cell lines. Using PCR Western blot, upregulation was observed inhibit EMT progression. Similarly, gain- loss-of-function revealed ectopic overexpression inhibited migration by transwell methods cells. RESULTS Our results acted as an inhibitor JEG-3 JAR cells; due its mediation transforming growth factor-β (TGFβ) signaling pathway, responsible for EMT. microarray analysis demonstrated interacted 3' untranslated region TGFβR2 mRNA, then blot dual-luciferase reporter gene assays verified interaction. CONCLUSION findings suggest partly accounts downregulation leads restraint migration. may therefore serve valuable potential target treatment