Distinctive protein expression patterns of the strain Brevundimonas sp. ZF12 isolated from the aqueous zone containing high levels of radiation to cadmium-induced stress

作者: Nasrin Masoudzadeh , Leila Alidoust , Nima Samie , Hamidreza Hajfarajollah , Hakimeh Sharafi

DOI: 10.1016/J.JBIOTEC.2014.05.028

关键词: MetabolismBiochemistryCadmiumBrevundimonasProteomicsOxidative stressStrain (chemistry)DNA repairBiologyPolyacrylamide gel electrophoresis

摘要: In the current study, different protein expression profiles in a strain Brevundimonas sp. ZF12, isolated from aqueous zone containing high levels of radiation, were characterized following exposure to cadmium (II) using proteomic strategy. order gain deeper understanding cellular events that allow this survive and undergo adaptation sorption, was tested under three experimental conditions 5, 10 30 ppm ions stress. Two-dimensional polyacrylamide gel electrophoresis mass spectrometry used identify differentially expressed proteins 20 spots successfully identified by MS/MS analysis. These are involved DNA repair protection, amino acid metabolism, nucleotide energy homeostasis, oxidative stress response, redox folding heat-shock response. The results obviously indicate ZF12 tends endure modification many aspects its physiology metabolism.

参考文章(47)
Elena Maestri, Natalya Klueva, Carla Perrotta, Mariolina Gulli, Henry T. Nguyen, Nelson Marmiroli, Molecular genetics of heat tolerance and heat shock proteins in cereals. Plant Molecular Biology. ,vol. 48, pp. 667- 681 ,(2002) , 10.1023/A:1014826730024
D. Rickwood, B. D. Hames, Gel electrophoresis of proteins: a practical approach : IRL. ,(1981)
Junichi Fujii, Shingo Matsuki, Tatsuya Ishii, Yoshihito Iuchi, Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian Journal of Andrology. ,vol. 5, pp. 231- 242 ,(2003)
Ilaria Marinoni, Simona Nonnis, Carmine Monteferrante, Peter Heathcote, Elisabeth Härtig, Lars H. Böttger, Alfred X. Trautwein, Armando Negri, Alessandra M. Albertini, Gabriella Tedeschi, Characterization of l-aspartate oxidase and quinolinate synthase from Bacillus subtilis FEBS Journal. ,vol. 275, pp. 5090- 5107 ,(2008) , 10.1111/J.1742-4658.2008.06641.X
Jenny Schelin, Fredrik Lindmark, Adrian K. Clarke, The clpP multigene family for the ATP-dependent Clp protease in the cyanobacterium Synechococcus. Microbiology. ,vol. 148, pp. 2255- 2265 ,(2002) , 10.1099/00221287-148-7-2255
HC Schröder, HMA Hassanein, S Lauenroth, C Koziol, TA-AA Mohamed, M Lacorn, H Steinhart, R Batel, WEG Müller, Induction of DNA strand breaks and expression of HSP70 and GRP78 homolog by cadmium in the marine sponge Suberites domuncula. Archives of Environmental Contamination and Toxicology. ,vol. 36, pp. 47- 55 ,(1999) , 10.1007/S002449900441
J.L Shepard, B Olsson, M Tedengren, B.P Bradley, Protein expression signatures identified in Mytilus edulis exposed to PCBs, copper and salinity stress Marine Environmental Research. ,vol. 50, pp. 337- 340 ,(2000) , 10.1016/S0141-1136(00)00065-9
Masoumeh Yousefi-Nejad, Hossein Naderi- Manesh, Khosro Khajeh, Proteomics of early and late cold shock stress on thermophilic bacterium, Thermus sp. GH5. Journal of Proteomics. ,vol. 74, pp. 2100- 2111 ,(2011) , 10.1016/J.JPROT.2011.05.037