Differential Effects of Fondaparinux and Bemiparin on Angiogenic and Vasculogenesis-like processes

摘要: article Introduction: Conventional therapy for venous thromboembolism or acute coronary syndrome involves the administration of glycoanticoagulants (heparins) oligosaccharides (fondaparinux). We evaluated effects such drugs on angiogenesis and vasculogenesis-like models. Materials Methods: Human umbilical vein endothelial cells human progenitor were treated with bemiparin, fondaparinux unfractionated heparin, at concentrations reflecting doses used in clinical practice. After 24 h, cell viability, proliferation, tubule formation angiogenic molecular mechanisms, as activation serine/threonine kinase AKT, assessed. In vivo was studied using a Matrigel sponge assay mice. Results: Bemiparin gave significant decrease vitro shown by reduction endothe- lial network, while both heparin did not show any effect. assays invasion mice, able to stimulate angiogen- esis and, conversely, bemiparin inhibited angiogenesis. Furthermore, caused an model, demonstrated network after co-seeding cells. addition, but increase AKT phosphorylation. Conclusions: experiments, only drug anti-angiogenic vasculogenic-like effect, showed trend affected model. Notably, experiments corroborated these data. Such results are important choice patient-tailored therapy.