Hepatitis C Virus Infection Induces Inflammatory Cytokines and Chemokines Mediated by the Cross Talk between Hepatocytes and Stellate Cells

摘要: Inflammatory cytokines and chemokines play important roles in inflammation during viral infection. Hepatitis C virus (HCV) is a hepatotropic RNA that closely associated with chronic liver inflammation, fibrosis, hepatocellular carcinoma. During the progression of HCV-related diseases, hepatic stellate cells (HSCs) contribute to inflammatory response triggered by HCV However, underlying molecular mechanisms mediate HSC-induced infection are not fully understood. By coculturing HSCs HCV-infected hepatocytes vitro, we found stimulated hepatocytes, leading expression proinflammatory such as interleukin-6 (IL-6), IL-8, macrophage protein 1α (MIP-1α), MIP-1β. Moreover, this effect was mediated IL-1α, which secreted HSCs. enhanced production CCAAT/enhancer binding (C/EBP) β mRNA, HSC-dependent IL-1α contributed stimulation C/EBPβ target hepatocytes. Consistent result, knockdown mRNA for resulted decreased after addition HSC conditioned medium. Induction medium required yet be identified postentry event productive The cross talk between key feature inflammation-mediated, diseases.