Polymethacrylate monolithic columns for hydrophilic interaction liquid chromatography prepared using a secondary surface polymerization

摘要: Zwitterionic methacrylate based polymeric monolithic columns were prepared in two-step polymerizations, with reduced polymerization times. Characteristic properties such as hydrodynamic permeability, porosity, retention factors, and pore size distribution charts used for column evaluation. A scaffold was fabricated by of poly(lauryl methacrylate-co-tetraethyleneglycol dimethacrylate) without further modification a support poly(N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine-co-bisphenol glycerolate second monolith layer zwitterionic functionality, HILIC separations. An additional internal structure formed the layer. The fabrication procedure reproducible RSD<5%. Field emission scanning electron microscopy has also been to investigate morphology, using novel technique where material is imaged directly, coverage conducting film or particles. new polar separations phenolic acids, flavones, nucleosides, bases nucleic similar efficiencies but different selectivities recently single step polymerization.