Epitope mapping and identification of amino acids critical for mouse IgG-binding to linear epitopes on Gly m Bd 28K.
作者： Jun Xi , Huili Yan
DOI: 10.1080/09168451.2016.1165604
关键词: Epitope mapping 、 IgG binding 、 Alanine scanning 、 Monoclonal antibody 、 Amino acid 、 Peptide sequence 、 Molecular biology 、 Immunoglobulin G 、 Epitope 、 Chemistry
摘要: Gly m Bd 28K is one of the major allergens in soybeans, but there limited information on its IgG-binding epitopes. Thirty-four overlapping peptides that covered entire sequence were synthesized, and 3 monoclonal antibodies against utilized to identify regions 28K. Three dominant corresponding (28)GDKKSPKSLFLMSNS(42)(G28-S42), (56)LKSHGGRIFYRHMHI(70)(L56-I70), (154)ETFQSFYIGGGANSH(168)(E154-H168) recognized. L56-I70 most important epitope, a competitive ELISA indicated it could inhibit binding antibody protein. Alanine scanning documented F64, Y65, R66 critical amino acids this epitope. Two bioinformatics tools, ABCpred BepiPred, used predict epitopes 28K, predictions compared with we had located by antibodies.
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