Lipid composition of Zymomonas mobilis: effects of ethanol and glucose.

摘要: Zymomonas mobilis is an alcohol-tolerant microorganism which potentially useful for the commercial production of ethanol. This organism was found to contain cardiolipin, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylcholine as major phospholipids. Vaccenic acid most abundant fatty acid, with lesser amounts myristic, palmitic, palmitoleic acids. No branched-chain or cyclopropane acids were found. Previous studies in our laboratory have shown that ethanol induces synthesis phospholipids enriched vaccenic Escherichia coli (L. O. Ingram, J. Bacteriol. 125:670-678, 1976). The composition Z. mobilis, obligately ethanol-producing microorganism, represents extreme trend observed E. coli. In over 75% acyl chains polar membrane lipids. Glucose had no effect on mobilis. However, both glucose caused a decrease phosphatidylethanolamine phosphatidylglycerol increase cardiolipin phosphatidylcholine. Ethanol also dose-dependent reduction lipid-to-protein ratios crude membranes. lipid may represent evolutionary adaptation survival presence Images