Tetraspanin CD151 maintains vascular stability by balancing the forces of cell adhesion and cytoskeletal tension.
作者： Feng Zhang , Jarett E. Michaelson , Simon Moshiach , Norman Sachs , Wenyuan Zhao
DOI: 10.1182/BLOOD-2011-03-339531
关键词: Vascular permeability 、 Cell adhesion 、 Biology 、 Cytoskeleton 、 Endothelial stem cell 、 Cell-matrix adhesion 、 Rac GTP-Binding Proteins 、 RHOA 、 Vascular endothelial growth factor A 、 Cell biology
摘要: Tetraspanin CD151 is highly expressed in endothelial cells and regulates pathologic angiogenesis. However, the mechanism by which promotes vascular morphogenesis whether engages other functions are unclear. Here we report that required for maintaining capillary-like structures formed vitro integrity of cell-cell cell-matrix contacts vivo. In addition, permeability markedly enhanced absence CD151. As a global regulator adhesions, needed optimal various cell adhesion proteins. The loss elevates actin cytoskeletal traction up-regulating RhoA signaling diminishes cortical meshwork down-regulating Rac1 activity. inhibition or activation cAMP stabilizes CD151-silenced -null structure morphogenesis. Together, our data demonstrate maintains stability promoting especially adhesion, confining tension.
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bloodjournal.org参考文章(51)
Krister Wennerberg, R Garcia-Mata, K Wennerberg, WT Arthur, NK Noren, SM Ellerbroek, K Burridge, None, Analysis of activated GAPs and GEFs in cell lysates. Methods in Enzymology. ,vol. 406, pp. 425- 437 ,(2006) , 10.1016/S0076-6879(06)06031-9
Gavin Thurston, John S. Rudge, Ella Ioffe, Hao Zhou, Leorah Ross, Susan D. Croll, Nicole Glazer, Jocelyn Holash, Donald M. McDonald, George D. Yancopoulos, Angiopoietin-1 protects the adult vasculature against plasma leakage. Nature Medicine. ,vol. 6, pp. 460- 463 ,(2000) , 10.1038/74725
J. Lammerding, A. R. Kazarov, H. Huang, R. T. Lee, M. E. Hemler, Tetraspanin CD151 regulates α6β1 integrin adhesion strengthening Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 7616- 7621 ,(2003) , 10.1073/PNAS.1337546100
M LEMONS, M CONDIC, Combined integrin activation and intracellular cAMP cause Rho GTPase dependent growth cone collapse on laminin-1 Experimental Neurology. ,vol. 202, pp. 324- 335 ,(2006) , 10.1016/J.EXPNEUROL.2006.06.008
Ingrid Stuiver, Timothy E. O'Toole, Regulation of integrin function and cellular adhesion. Stem Cells. ,vol. 13, pp. 250- 262 ,(1995) , 10.1002/STEM.5530130306
Anjaruwee S. Nimnual, Laura J. Taylor, Dafna Bar-Sagi, Redox-dependent downregulation of Rho by Rac Nature Cell Biology. ,vol. 5, pp. 236- 241 ,(2003) , 10.1038/NCB938
Guillaume Mandicourt, Sandra Iden, Klaus Ebnet, Michel Aurrand-Lions, Beat A. Imhof, JAM-C Regulates Tight Junctions and Integrin-mediated Cell Adhesion and Migration Journal of Biological Chemistry. ,vol. 282, pp. 1830- 1837 ,(2007) , 10.1074/JBC.M605666200
Molly A. Thoreson, Panos Z. Anastasiadis, Juliet M. Daniel, Reneé C. Ireton, Margaret J. Wheelock, Keith R. Johnson, Diana K. Hummingbird, Albert B. Reynolds, Selective Uncoupling of P120 ctn from E-Cadherin Disrupts Strong Adhesion Journal of Cell Biology. ,vol. 148, pp. 189- 202 ,(2000) , 10.1083/JCB.148.1.189
Z.-J. Su, C. N. Hahn, G. J. Goodall, N. M. Reck, A. F. Leske, A. Davy, G. Kremmidiotis, M. A. Vadas, J. R. Gamble, A vascular cell-restricted RhoGAP, p73RhoGAP, is a key regulator of angiogenesis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 12212- 12217 ,(2004) , 10.1073/PNAS.0404631101
George E. Plopper, Janice L. Huff, Will L. Rust, Martin A. Schwartz, Vito Quaranta, Antibody-induced activation of beta1 integrin receptors stimulates cAMP-dependent migration of breast cells on laminin-5. Molecular Cell Biology Research Communications. ,vol. 4, pp. 129- 135 ,(2000) , 10.1006/MCBR.2001.0267