Effect of pH on the metabolic flux of Klebsiella oxytoca producing 2,3-butanediol in continuous cultures at different dilution rates.

摘要: The efficiency of the bioconversion process and achievable end-product concentration decides economic feasibility microbial 2,3-butanediol (2,3-BDO) production. In 2,3-BDO production, optimization culture condition is required for cell growth metabolism. Also, pH an important factor that influences performance. For different microorganisms substrates, it has been shown distribution metabolites in fermentation greatly affected by pH, optimum production seems dependently linked to particular strain substrate employed. Quantification analysis intracellular metabolic flux (MFA) were used investigate effect on Klebsiella oxytoca producing other organic acids. main objectives MFA are estimation fluxes identification rate-limiting step key enzymes. This study was conducted under continuous aerobic conditions at dilution rates (0.1, 0.2, 0.3 h−1) values (pH 5.5 7.0) steady-state experimental data. order obtain distribution, extracellular specific calculated from data using network model K. oxytoca. Intracellular metabolite profiles generated ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry.