Modulation of cellular antioxidant defense activities by sodium arsenite in human fibroblasts.
作者： Te -Chang Lee , I -Ching Ho
关键词: Sodium azide 、 Biochemistry 、 Dichlorofluorescein 、 Glutathione 、 Sodium arsenite 、 Sodium 、 Arsenite 、 Antioxidant 、 Heme oxygenase 、 Chemistry
摘要: Many studies have shown that oxygen radicals can be produced during arsenic metabolism. We report here in human fibroblasts (HFW cells) sodium arsenite exposure caused increased formation of fluorescent dichlorofluorescein (DCF) by oxidation the nonfluorescent form. The enhanced DCF fluorescence was inhibited a radical scavenger, butylated hydroxytoluene. effects treatment on cellular antioxidant activities were then examined. Treatment HFW cells with resulted significant increase heme oxygenase activity and ferritin level. Sodium arsenite-enhanced synthesis co-treatment antioxidants azide dimethyl sulfoxide. Furthermore, did not apparently affect glucose-6-phosphate dehydrogenase activity, but significantly glutathione levels superoxide dismutase slightly decreased peroxidase catalase activity. toxicity partly reduced addition to culture medium. These results imply enhance oxidative stress cells.
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