Flow cytometric detection of apoptotic bone marrow cells with fractional DNA content after application of WR-2721, cyclophosphamide, cisplatin, and exposure of mice to gamma rays.
作者： L Mazur , A Czyzewska , M Bochenek
DOI: 10.1191/0960327102HT261OA
关键词: Bone marrow 、 Amifostine 、 Nitrogen mustard 、 Apoptosis 、 Cisplatin 、 Flow cytometry 、 Cell cycle 、 Immunology 、 Biology 、 Cancer research 、 Cyclophosphamide
摘要: Little is known about the mechanisms of apoptosis triggered in normal cells haemopoietic system by aminothiol WR-2721 (Amifostine), chemotherapeutic drugs, and ionizing radiation; thus, present study was undertaken to evaluate effects WR-2721, cyclophosphamide (CP), cisplatin (CDDP), 60Co gamma rays on induction apoptotic DNA degradation bone marrow cells. Adult male Swiss mice were treated with (400 mg/kg b.wt.), CP (200 CDDP (10 mg/ kg exposed 6 Gy rays. Alterations number fractional content also cell cycle position non-apoptotic determined at 7 24 hours after treatment these agents, using flow cytometric assay controlled extraction low-MW from The drugs affected mou...
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sci-hub.se 参考文章(68)
H Bonner, L M Shaw, R Lieberman, Pharmacokinetic profile of amifostine Seminars in Oncology. ,vol. 23, pp. 18- 22 ,(1996)
Malcolm J. Moore, Clinical pharmacokinetics of cyclophosphamide. Clinical Pharmacokinectics. ,vol. 20, pp. 194- 208 ,(1991) , 10.2165/00003088-199120030-00002
Arlene Hwang, Ruth J. Muschel, Radiation and the G2 Phase of the Cell Cycle Radiation Research. ,vol. 150, ,(1998) , 10.2307/3579808
Zbigniew Darzynkiewicz, Frank Traganos, Measurement of apoptosis Apoptosis. ,vol. 62, pp. 33- 73 ,(1998) , 10.1007/BFB0102305
C M Kurbacher, P K Mallmann, Chemoprotection in anticancer therapy: the emerging role of amifostine (WR-2721). Anticancer Research. ,vol. 18, pp. 2203- 2210 ,(1998)
Rosemary B. Evans-Storms, John A. Cidlowski, Molecular Regulation of Apoptosis Humana Press, Totowa, NJ. pp. 415- 427 ,(2000) , 10.1007/978-1-59259-032-2_25
Principles of molecular regulation Humana Press. ,(2000) , 10.1007/978-1-59259-032-2
Zbigniew Darzynkiewicz, Xun Li, Tomoyuki Murakami, Shinsuke Hara, Yoshihiro Numa, Flow Cytometric Analysis of Cell Death : A Critical Review Cytometry research : 日本サイトメトリー学会機関誌. ,vol. 5, pp. 1- 8 ,(1995)
Jir̆í Kozelka, Jean-Claude Chottard, How does cisplatin alter DNA structure? A molecular mechanics study on double-stranded oligonucleotides. Biophysical Chemistry. ,vol. 35, pp. 165- 178 ,(1990) , 10.1016/0301-4622(90)80006-S
Peter W. Mesner, I. Imawati Budihardjo, Scott H. Kaufmann, Chemotherapy-Induced Apoptosis Advances in pharmacology. ,vol. 41, pp. 461- 499 ,(1997) , 10.1016/S1054-3589(08)61069-8