Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna

摘要: The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this establishing combinations factors, including fusions between conventional factors with transactivation domains, optimized various types cells. More specifically, exemplary disclosed herein can be used creating from mammalian cell types, human fibroblasts. Exemplary feeder-free derivation synthetic messenger RNA are also disclosed.