Bril: A novel bone-specific modulator of mineralization

摘要: In the course of attempting to define bone secretome using a signal-trap screening approach, we identified gene encoding small membrane protein novel osteoblasts. Although previously in silico as ifitm5, no localization or functional studies had been undertaken on this gene. We characterized expression patterns and vitro vivo assessed its role matrix mineralization vitro. The specificity shown led us rename restricted ifitm-like (Bril). Bril encodes 14.8-kDa 134 amino acid with two transmembrane domains. Northern blot analysis showed bone-specific other embryonic adult tissues. situ hybridization immunohistochemistry mouse embryos localized developing bone. Screening cell lines be highest osteoblasts, associated onset maturation/mineralization, suggesting formation. Functional evidence was by adenovirus-mediated overexpression lentivirus-mediated shRNA knockdown Elevated resulted dose-dependent increases UMR106 rat primary Conversely, MC3T3 osteoblasts reduced mineralization. Thus, osteoblast mineralization, possibly identifying new regulatory pathway