Rapid and Dynamic Alternative Splicing Impacts the Arabidopsis Cold Response Transcriptome

摘要: Plants have adapted to tolerate and survive constantly changing environmental conditions by reprogramming gene expression The dynamics of the contribution alternative splicing (AS) stress responses are unknown. RNA-sequencing a time-series Arabidopsis thaliana plants exposed cold determines timing significant AS changes. This shows massive rapid response with coincident waves transcriptional activity occurring in first few hours temperature reduction further throughout cold. In particular, hundreds genes showed changes due rapidly (“early AS” genes); these included numerous novel cold-responsive transcription factors factors/RNA binding proteins regulated only AS. speed sensitivity small some suggest that fine-tuning via pathways contributes thermo-plasticity expression. Four early regulatory been shown previously be required for freezing tolerance acclimation; we provide evidence fifth gene, U2B”-LIKE. Such likely drive cascades downstream that, alongside transcription, modulate transcriptome together govern physiological survival low temperature.