Novel Vibrio spp. Strains Producing Omega-3 Fatty Acids Isolated from Coastal Seawater.

摘要: Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), such as eicosapentaenoic acid (EPA) (20:5n-3) and docosahexaenoic (DHA) (22:6n-3), are considered essential for human health. Microorganisms the primary producers of omega-3 in marine ecosystems, representing a sustainable source these lipids, an alternative to fish industry. Some bacteria can produce LC-PUFAs de novo via Polyunsaturated Fatty Acid (Pfa) synthase/ Polyketide Synthase (PKS) pathway, which does not require desaturation elongation saturated acids. Cultivation-independent surveys have revealed that diversity microorganisms harboring molecular marker pfa gene cluster (i.e., pfaA-KS domain) is high their potential distribution systems widespread, from surface seawater sediments. However, isolation PUFA waters has been typically restricted deep or cold environments. Here, we report phenotypic genotypic screening identification free-living bacterial strains isolated 5, 500, 1000 m coastal Bay Biscay (Spain). We further measured EPA production pelagic Vibrio sp. collected at three different depths. EPA-producers non-producers were simultaneously same water samples shared percentage identity 16S rRNA genes, supporting view be horizontally transferred. Within EPA-producers, found intraspecific variation levels synthesis isolates genetic variants domain. The maximum was strain depth (average 4.29% ± 1.07 total 10 °C, without any optimization culturing conditions).