Phylogenetically diverse groups of Bradyrhizobium isolated from nodules of Crotalaria spp., Indigofera spp., Erythrina brucei and Glycine max growing in Ethiopia.

摘要: Ethiopian Bradyrhizobium strains isolated from root nodules of Crotalaria spp., Indigofera Erythina brucei and soybean (Glycine max) represented genetically diverse phylogenetic groups the genus Bradyrhizobium. Strains were characterized using amplified fragment length polymorphism fingerprinting technique (AFLP) multilocus sequence analysis (MLSA) core symbiotic genes. Based on analyses concatenated recA-glnII-rpoB-16S rRNA genes sequences, distributed into fifteen under B. japonicum elkanii super clades. Some isolates belonged to species yuanmingense, type I. However, majority unnamed genospecies these, two unique lineages that most likely represent novel identified among strains. The nodulation nodA gene revealed all sub-clade III.3. further classified 14 together with Africa, as well some originating other tropical subtropics regions. also clustered in nodY/K phylogeny similarly tree. nifH phylogenies generally congruent phylogeny, supporting monophyletic origin partially inferred reflecting obtained their vertically ancestor horizontally more distantly related species.