Chondrogenic Differentiation of Cultured Human Mesenchymal Stem Cells from Marrow
作者： Alastair M. Mackay , Stephen C. Beck , J. Mary Murphy , Frank P. Barry , Clinton O. Chichester
关键词: Cartilage metabolism 、 Stem cell transplantation for articular cartilage repair 、 Cartilaginous Tissue 、 Mesenchymal stem cell 、 Cell biology 、 Aggrecan 、 Bone marrow 、 Immunology 、 Chondrogenesis 、 Extracellular matrix 、 Chemistry
摘要: In the adult human, mesenchymal stem cells (MSCs) resident in bone marrow retain capacity to proliferate and differentiate along multiple connective tissue lineages, including cartilage. this study, culture-expanded human MSCs (hMSCs) of 60 donors were induced express morphology gene products chondrocytes. Chondrogenesis was by culturing hMSCs micromass pellets presence a defined medium that included 100 nM dexamethasone 10 ng/ml transforming growth factor-beta(3) (TGF-beta(3)). Within 14 days, secreted an extracellular matrix incorporating type II collagen, aggrecan, anionic proteoglycans. could be further differentiated hypertrophic state addition 50 thyroxine, withdrawal TGF-beta(3), reduction concentration 1 nM. Increased understanding induction chondrogenic differentiation should lead progress defining mechanisms responsible for generation cartilaginous tissues, their maintenance, regeneration.
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