Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.

摘要: The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye used here as a buffer additive for dynamic modification of the sample proteins microorganisms. values points labeled bioanalytes were calculated use fluorescent pI markers found comparable native compounds. mixed cultures microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis 4418, Proteus vulgaris, Enterococcus faecalis 4224, Stenotrophomonas maltophilia, strains yeast cells, Candida albicans 8180, krusei, parapsilosis, glabrata, tropicalis, Saccharomyces cerevisiae reproducibly focused separated by suggested technique. Using UV excitation on-column detection, minimum detectable amount wa...