In Vitro Culture of Lowbush Blueberry (Vaccinium angustifolium Ait.)
作者： Samir C. Debnath
关键词: Explant culture 、 Lowbush blueberry 、 Biology 、 Basal shoot 、 Shoot 、 Tissue culture 、 Micropropagation 、 Botany 、 Horticulture 、 Zeatin 、 Vaccinium
摘要: SUMMARY Cultures of three lowbush blueberry (Vaccinium angustifolium Ait.) clones collected from natural stands in Newfoundland were established vitro on a modified cranberry (V. macrocarpon tissue culture medium containing zeatin (5 μM) or N6-[2-isopentenyl]adenine (2iP) (10 μM). Shoot proliferation with respect to shoot number per explant differed among at various concentrations over two periods. Best total was obtained when basal nodal segments cultured the supplemented 2-4 μM zeatin. In another experiment, explants more productive than tips. Shoots growing for 12 weeks media that contained 4 occasionally produced adventitious masses, which appeared arise dense calli base shoots medium. The lower concentration sucrose and irradiance improved vigor compared control treatments (30 g L_1 an...
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