Organization of the yeast ribosomal RNA gene cluster via cloning and restriction analysis.
作者： K Nath , A P Bollon
DOI: 10.1016/S0021-9258(17)39994-5
关键词: Restriction fragment 、 Restriction enzyme 、 Restriction fragment length polymorphism 、 Biology 、 Genomic library 、 Genetics 、 Molecular biology 、 Restriction map 、 Restriction site 、 EcoRI 、 Amplified fragment length polymorphism
摘要: Abstract The restriction endonuclease EcoR1 cleaves Saccharomyces cerevisiae DNA, which codes for ribosomal RNA (rRNA), into seven fragments, A second endonuclease, HindIII, the same yeast DNA two fragments. These enzymes each yield segments that total about 5.9 megadaltons. "repeat unit" of genes coding rRNA is thus megadaltons or 9000 base pairs long. HindIII-cleaved fragments as well one EcoR1-cleaved were purified and amplified by cloning in Escherichia coli. Three EcoR1-generated could then be ordered treating cloned HindIII with EcoR1. This led assignment sites. various hybridized directly from gel utilizing 32P-labeled 5 S, 5.8 18 25 S rRNA. Identification to final ordering gene adjacent 35 precursor groups occur a cluster following sequence: [5 S-spacer]-[spacer-18 S-5.8 S-25 S-spacer]-[spacer-5 S]. actual map presented.
sci-hub.st 参考文章(30)
Arthur P. Bollon, Regulation of the ilv 1 multifunctional gene in Saccharomyces cerevisiae. Molecular Genetics and Genomics. ,vol. 142, pp. 1- 12 ,(1975) , 10.1007/BF00268750
Gerald M. Rubin, The Nucleotide Sequence of Saccharomyces cerevisiae 5.8 S Ribosomal Ribonucleic Acid Journal of Biological Chemistry. ,vol. 248, pp. 3860- 3875 ,(1973) , 10.1016/S0021-9258(19)43814-3
Kamalendu Nath, Jerard Hurwitz, Covalent Attachment of Polyribonucleotides to Polydeoxyribonucleotides Catalyzed by Deoxyribonucleic Acid Ligase Journal of Biological Chemistry. ,vol. 249, pp. 3680- 3688 ,(1974) , 10.1016/S0021-9258(19)42528-3
Øystein Karijord, Tordis B. Øyen, Study of a haploid yeast strain with an unusually high rDNA content: II. Fractionation of the DNA in preparative Ag+-Cs2 SO4 density gradients Biochimica et Biophysica Acta. ,vol. 383, pp. 255- 265 ,(1975) , 10.1016/0005-2787(75)90054-4
N R Pace, Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriological Reviews. ,vol. 37, pp. 562- 603 ,(1973) , 10.1128/BR.37.4.562-603.1973
R K Mortimer, D C Hawthorne, Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. ,vol. 74, pp. 33- 54 ,(1973) , 10.1093/GENETICS/74.1.33
David B. Kaback, Harlyn O. Halvorson, Gerald M. Rubin, Location and magnification of 5 S RNA genes in Saccharomyces cerevisiae Journal of Molecular Biology. ,vol. 107, pp. 385- 390 ,(1976) , 10.1016/S0022-2836(76)80012-5
Ueli Schibler, Toni Wyler, Otto Hagenbüchle, Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution Journal of Molecular Biology. ,vol. 94, pp. 503- 517 ,(1975) , 10.1016/0022-2836(75)90217-X
Kamalendu Nath, Arthur P. Bollon, Characterization of yeast ribosomal DNA fragments generated by EcoR1 restriction endonuclease. Molecular Genetics and Genomics. ,vol. 147, pp. 153- 168 ,(1976) , 10.1007/BF00267567
Richard A. Kramer, John R. Cameron, Ronald W. Davis, Isolation of bacteriophage λ containing yeast ribosomal RNA genes: Screening by in situ RNA hybridization to plaques Cell. ,vol. 8, pp. 227- 232 ,(1976) , 10.1016/0092-8674(76)90006-4