Induction of gynogenesis in the turbot (Scophthalmus maximus):: Effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age

摘要: Abstract Fish in which gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are homogametic sex, they usually females. Because turbot grow faster than males, production of all-female populations is highly desirable. The sperm poor quality and its larvae small fragile. These circumstances represent a challenge for induction turbot. As first step towards this goal, effective conditions through UV irradiation followed by cold shock were established. When diluted 1:10 with Ringer-200 saline solution placed thin layer (∼0.3 mm), dose-dependent effect light on motility was found. dose at both amount motile duration reduced to 50% original value (ID 50 ) ∼28,000 erg mm −2 . A typical Hertwig elicited 30,000 resulting embryos exhibited “haploid syndrome” died shortly after hatching. Application (−1 0°C 25 min starting 6.5 fertilization) activated eggs UV-irradiated (30,000 (1:10) sperms restored diploidy resulted gynogenetic diploids (2 n =44 chromosomes). used pilot-scale experiment found inducing ∼47,000 eggs. rate 100% as verified an analysis microsatellite DNA markers. Survival gynogenetics approximately 10% 6 months age, although growth similar during period. If species turns out female homogamety, case most pleuronectiformes examined so far, method presented here necessary economically important species.