[Human monoclonal antibodies with human-mouse hybridomas].

摘要: Our trial to produce human monoclonal antibodies with human-mouse hybridomas is described. Human spleen cells, in comparison peripheral blood lymphocytes, bone marrow cells and lymphnode seem be the best partner fuse myeloma line NS-1 obtain hybridomas. About sixty percent of derived from at first Ig which most cases belong either IgG or IgM. Fifty had initially produced continued do so after 4 months. One clones reacted a cell surface antigen expressed on three out 5 B lines, 14 38 Epstein-Barr virus transformed lines null type lymphocytic leukemia obtained one patient 34 tested. Other normal malignant hematopoietic nonhematopoietic examined were negative. The does not related EBNA, early VCA EB virus, certain haplotype specificity HLA-A, B, C, DR loci. Reports by others production similar approaches also reviewed.