Hyperspectral stimulated Raman microscopy with two fiber laser sources

摘要: A fast all fiber based setup for stimulated Raman microscopy on a rapidly wavelength swept cw-laser is presented. The applied Fourier domain mode locked (FDML) laser ring laser, providing continuously changing output over time. This source allows us to change the and, thereby energy difference with respect single color pump laser. master oscillator power amplifier amplified diode and shifter. By controlled variation of relative timing between probe an arbitrary waveform generator, signals are encoded in time they directly acquired synchronized, analog-to-digital converter. capable acquiring high resolution spectra (up 0.5 cm-1) shot noise limited sensitivity broadband (750 cm-1 3150 spectral region. Here, we show performance this system imaging CH-stretch region around 3000 fingerprint 1600 cm-1. We present hyperspectral images plant stem slice molecular contrast lignin lipid representative as well PS (polystyrene) PMMA (poly(methyl methacrylate) beads acquisition speed 18 µs per point.